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Article

Introduction of conditional lethal amber mutations in Escherichia coli.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2008

Herring Christopher D

Abstract excerpt

A method is described for generating conditional lethal mutations in essential genes in Escherichia coli. In this procedure, amber stop codons are introduced as "tagalong" mutations in the flanking DNA of a downstream antibiotic-resistance marker by lambda Red recombination. The marker is removed by expression of I-SceI homing endonuclease, leaving a markerless mutation. The mutants then depend upon expression of...

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Share to Anti-Bacterial Agents
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to RNA, Transfer, Amino Acyl

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Identifiers

PMID: 18392977
DOI: 10.1007/978-1-59745-321-9_21

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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