In vivo selection of conditional-lethal mutations in the gene encoding elongation factor G of Escherichia coli.

doi:10.1128/jb.176.1.123-129.1994

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

In vivo selection of conditional-lethal mutations in the gene encoding elongation factor G of Escherichia coli.

Journal of bacteriology - 1 Jan 1994

Hou Y, Lin Y P, Sharer J D, March P E

Abstract excerpt

The ribosome translocation step that occurs during protein synthesis is a highly conserved, essential activity of all cells. The precise movement of one codon that occurs following peptide bond formation is regulated by elongation factor G (EF-G) in eubacteria or elongation factor 2 (EF-2) in euk...

Topics

Alleles
Amino Acid Sequence
Cell Division
Cloning, Molecular
Enzyme Induction
Escherichia coli
Genes, Bacterial
Genes, Lethal
Genetic Complementation Test
Hot Temperature
Kanamycin Resistance
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Peptide Elongation Factor G

Peptide Elongation Factors

Protein Biosynthesis

Selection, Genetic

Sequence Analysis, DNA

Source

Open in PubMed Open full text

Identifiers

PMID: 8282687
DOI: 10.1128/jb.176.1.123-129.1994
PMCID: PMC205022

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8282687-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

In vivo selection of conditional-lethal mutations in the gene encoding elongation factor G of Escherichia coli.PMID 8282687Read ↗

Alterations in the peptidyltransferase and decoding domains of ribosomal RNA suppress mutations in the elongation factor G gene.Semantic proximity Read ↗

Novel mutants of elongation factor G.Semantic proximity Read ↗

Molecular analysis of fusidic acid resistance in Staphylococcus aureusSemantic proximity Read ↗

Staphylococcus aureus elongation factor G--structure and analysis of a target for fusidic acid.Semantic proximity Read ↗

Modulation of Global Transcriptional Regulatory Networks as a Strategy for Increasing Kanamycin Resistance of the Translational Elongation Factor-G Mutants in Escherichia coli.Semantic proximity Read ↗

Use of the Golden Gate Cloning Technique for Plasmid Construction to Generate Conditional Lethal Mutants in C. difficile.Semantic proximity Read ↗

Selection of Highly Expressed Gene Variants in Escherichia coli Using Translationally Coupled Antibiotic Selection Markers.Semantic proximity Read ↗

A central interdomain protein joint in elongation factor G regulates antibiotic sensitivity, GTP hydrolysis, and ribosome translocation.Semantic proximity Read ↗

Conditional lethal amber mutations in essential Escherichia coli genes.Semantic proximity Read ↗

Biological cost and compensatory evolution in fusidic acid-resistant Staphylococcus aureus.Semantic proximity Read ↗

Targeted introduction of a diphtheria toxin-resistant point mutation into the chromosomal EF-2 locus by in vivo homologous recombination.Semantic proximity Read ↗

Isolation and analysis of dominant secA mutations in Escherichia coli.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.