Article
A CRISPR-Based Method for Constructing Conditional Mutations of Essential Genes in Cyanobacteria.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2022
Zhang Ju-Yuan, Niu Tian-Cai, Lin Gui-Ming, Zhang Cheng-Cai
Abstract excerpt
Cyanobacteria, a group of diverse bacteria capable of oxygenic photosynthesis, are excellent models for investigating many important cellular processes, such as photosynthesis, nitrogen fixation, and prokaryotic cell differentiation. They also have great potential to become the next-generation cell factories for sustainable biosynthesis of valuable products. However, genetic manipulation in cyanobacteria is not...
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