Article
Efficient introduction of cloned mutant alleles into the Escherichia coli chromosome.
Journal of bacteriology - 1 Apr 1991
Kulakauskas S, Wikström P M, Berg D E
Abstract excerpt
An efficient method for moving mutations in cloned Escherichia coli DNA from plasmid vectors to the bacterial chromosome was developed. Cells carrying plasmids that had been mutated by the insertion of a resistance gene were infected with lambda phage containing homologous cloned DNA, and resulting lysates were used for transduction. Chromosomal transductants (recombinants) were distinguished from plasmid...
Topics
- Alleles
- Bacteriophage lambda
- Cloning, Molecular
- Escherichia coli
- Genes, Bacterial
- Mutagenesis, Insertional
- Plasmids
- Transduction, Genetic