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Article

A novel, simple, high-throughput method for isolation of genome-wide transposon insertion mutants of Escherichia coli K-12.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2008

Miki Takeyoshi, Yamamoto Yoshihiro, Matsuda Hideo

Abstract excerpt

We developed a novel, simple, high-throughput method for isolation of genome-wide transposon insertion mutants of Escherichia coli K-12. The basic idea of the method is to randomly disrupt the genes on the DNA fragments cloned on the Kohara library by inserting a mini-transposon first, and then transfer the disrupted genes from the lambda vector to the E. coli chromosome by homologous recombination. Using this...

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PMID: 18392969
DOI: 10.1007/978-1-59745-321-9_13

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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