Article
Constitutive expression of the mature array of neurofilament proteins by a CNS neuronal cell line.
Brain research - 6 Sept 1991
Lee H J, Elliot G J, Hammond D N, Lee V M, Wainer B H
Abstract excerpt
Neurofilament protein expression was examined immunochemically in a neuronal cell line derived from postnatal day 21 septal tissue. The SN48.1p cell line was found to constitutively synthesize an array of neurofilament proteins typical of a mature neuron. All three neurofilament subunits (NF-L, NF-M, and NF-H) as well as differentially phosphorylated isoforms (P-, P+, P++, and P ) of NF-M and NF-H were identified...
Topics
- Animals
- Antibodies
- Antibodies, Monoclonal
- Brain
- Brain Chemistry
- Cell Line
- Clone Cells
- Cytoskeleton
- Electrophoresis, Polyacrylamide Gel
- Fluorescent Antibody Technique
- GAP-43 Protein