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Article

Sugar binding to recombinant wild-type and mutant glucokinase monitored by kinetic measurement and tryptophan fluorescence.

The Biochemical journal - 15 Jul 2008

Zelent Bogumil, Odili Stella, Buettger Carol, Shiota Chiyo, Grimsby Joseph, Taub Rebecca, Magnuson Mark A, Vanderkooi Jane M, Matschinsky Franz M

Abstract excerpt

Tryptophan fluorescence was used to study GK (glucokinase), an enzyme that plays a prominent role in glucose homoeostasis which, when inactivated or activated by mutations, causes diabetes mellitus or hypoglycaemia in humans. GK has three tryptophan residues, and binding of D-glucose increases their fluorescence. To assess the contribution of individual tryptophan residues to this effect, we generated GST-GK [GK...

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Identifiers

PMID: 18370929
DOI: 10.1042/BJ20071718

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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