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Article

Tryptophan Fluorescence Yields and Lifetimes as a Probe of Conformational Changes in Human Glucokinase.

Journal of fluorescence - 1 Sept 2017

Zelent Bogumil, Bialas Chris, Gryczynski Ignacy, Chen Pan, Chib Rahul, Lewerissa Karina, Corradini Maria G, Ludescher Richard D, Vanderkooi Jane M, Matschinsky Franz M

Abstract excerpt

Five variants of glucokinase (ATP-D-hexose-6-phosphotransferase, EC 2.7.1.1) including wild type and single Trp mutants with the Trp residue at positions 65, 99, 167 and 257 were prepared. The fluorescence of Trp in all locations studied showed intensity changes when glucose bound, indicating that conformational change occurs globally over the entire protein. While the fluorescence quantum yield changes upon...

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Identifiers

PMID: 28432632
DOI: 10.1007/s10895-017-2099-x
PMCID: PMC6025808

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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