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Article

Conversion of a cyclodextrin glucanotransferase into an alpha-amylase: assessment of directed evolution strategies.

Biochemistry - 2 Oct 2007

Kelly Ronan M, Leemhuis Hans, Dijkhuizen Lubbert

Abstract excerpt

Glycoside hydrolase family 13 (GH13) members have evolved to possess various distinct reaction specificities despite the overall structural similarity. In this study we investigated the evolutionary input required to effeciently interchange these specificities and also compared the effectiveness of laboratory evolution techniques applied, i.e., error-prone PCR and saturation mutagenesis. Conversion of our model...

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Share to alpha-Amylases
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 17824673
DOI: 10.1021/bi701160h

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