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Article

Expression cloning of genes for GPI-anchor biosynthesis.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas - 1 Feb 1994

Kinoshita T, Takahashi M, Inoue N, Miyata T, Takeda J

Abstract excerpt

Cloning genes for glycosylphosphatidylinositol (GPI)-anchor biosynthesis is important to further understand its mechanisms and regulation. We have been using expression cloning methods in which a cDNA library was transfected into GPI-anchor-deficient mutant cells. The transfectants which restored surface expression of GPI-anchored proteins were isolated and the plasmids were rescued. In this way we previously...

Topics

Animals
Cells, Cultured
Cloning, Molecular
DNA, Complementary
Gene Expression Regulation
Gene Library
Genetic Vectors
Glycosylphosphatidylinositols
Hemoglobinuria, Paroxysmal
Herpesvirus 4, Human

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Humans

Mutation

Transfection

Source

Identifiers

PMID: 8081220

Genes

GPI

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8081220-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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