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Article

Isolation of temperature-sensitive yeast GPI-anchoring mutants.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas - 1 Feb 1994

Orlean P, Leidich S D, Drapp D A, Colussi P

Abstract excerpt

We are using a genetic approach to explore the synthesis and function of glycosylphosphatidylinositol (GPI). We have developed a novel strategy to isolate Saccharomyces cerevisiae mutants blocked in GPI anchoring by screening colonies of mutagenized yeast cells for those that fail to incorporate [3H]inositol into protein. Among our isolates are strains blocked in mannosylation of the GPI-anchorprecursor, and...

Topics

DNA, Complementary
Fungal Proteins
Genomic Library
Glycosylphosphatidylinositols
Mutation
Protein Precursors
Saccharomyces cerevisiae
Temperature

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 8081223

Genes

GPI

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8081223-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Isolation of temperature-sensitive yeast GPI-anchoring mutants.PMID 8081223Read ↗

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