Article
Spontaneous mutation at a 5-methylcytosine hotspot is prevented by very short patch (VSP) mismatch repair.
Genetics - 1 May 1991
Lieb M
Abstract excerpt
In many strains of Escherichia coli, the product of gene dcm methylates the internal cytosines in the sequence 5'CC(A or T)GG. Spontaneous deamination of 5-methylcytosine produces thymine which, if not corrected, can result in a transition mutation. 5-Methylcytosines in the lacI gene are hotspots for spontaneous C to T mutations. dcm is linked to vsr, a gene required for very short patch (VSP) repair. VSP repair...
Topics
- 5-Methylcytosine
- Bacteriophage lambda
- Base Sequence
- Cytosine
- DNA Mutational Analysis
- DNA Repair
- DNA, Bacterial
- DNA, Viral
- Escherichia coli
- Methylation
- Mutation