Article
An orphan DNA (cytosine-5-)-methyltransferase in Vibrio cholerae.
Microbiology (Reading, England) - 1 Apr 2006
Banerjee Sanjib, Chowdhury Rukhsana
Abstract excerpt
5-Methyl cytosine (m5C) was detected in genomic DNA of the enteric pathogen Vibrio cholerae by HPLC analysis and immunoblotting with m5C-specific antibody. Although cleavage with the restriction endonuclease EcoRII revealed the absence of a Dcm homologue in V. cholerae, analysis of the genome sequence indicated the presence of a gene, designated in this study as vchM, which encodes a DNA...
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