Synthesis and expression in E. coli of the gene for human tumor necrosis factor (h alpha TNF).

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Article

Synthesis and expression in E. coli of the gene for human tumor necrosis factor (h alpha TNF).

Archivum immunologiae et therapiae experimentalis - 1 Jan 1991

Kłysik J, Konarzewska-Zglińska A, Gałazka G, Uznański B, Okruszek A, Guga P, Wilk A, Koziołkiewicz M, Tchórzewski H, Zembala M

Abstract excerpt

A gene coding for human tumor necrosis factor (h alpha TNF) has been assembled by ligating short oligodeoxyribonucleotides and cloning into plasmid vectors. These oligonucleotides were prepared by the modified phosphoramidite methodology using isopropoxyacetyl (IPA) as a protecting group for exoa...

Topics

Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA
Escherichia coli
Gene Expression
Genetic Variation
Humans
Molecular Sequence Data
Recombinant Proteins
Tumor Necrosis Factor-alpha

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Open in PubMed

Identifiers

PMID: 1821611

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Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1821611-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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