Article
Methods of preparation of recombinant cytokine proteins.
Protein expression and purification - 1 Nov 1998
Klyushnichenko V E, Tikhonov R V, Pechenov S E, Yakimov S A, Shingarova L N, Korobko V G, Wulfson A N
Abstract excerpt
Two schemes for efficient and productive isolation for mutant human recombinant tumor necrosis factor-alpha (TNF-alpha R32H) from Escherichia coli cells were developed. The methods include membrane filtration, ion-exchange chromatography and gel filtration, and centrifugation with subsequent free-flow electrophoresis as an alternative procedure. The target product was obtained as active trimer with total yield...
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