Article
Expression and subcellular location of native and mutant hTNF alpha proteins in Escherichia coli.
FEMS microbiology letters - 1 Dec 1991
Gase K, Wagner B, Wagner M, Wollweber L, Behnke D
Abstract excerpt
Several mutant hTNF alpha genes were constructed by deletion and stepwise reconstitution of regions coding for C-terminal sequences. The mutant hTNF alpha proteins behaved differently from native hTNF alpha when expressed in Escherichia coli. They were either sensitive to proteolytic degradation or formed insoluble aggregates depending on the strains and conditions used for expression. By contrast, native hTNF...
Topics
- Amino Acid Sequence
- DNA Mutational Analysis
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Humans
- Immunohistochemistry
- Microscopy, Immunoelectron
- Molecular Sequence Data
- Mutation
- Recombinant Proteins