Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Constitutive versus thermoinducible expression of heterologous proteins in Escherichia coli based on strong PR,PL promoters from phage lambda

14 May 2003

Abstract excerpt

Constitutive and thermoinducible expression plasmids based on strong P(R),P(L) promoters from phage lambda were compared for production of TNF-alpha and its analogs under various conditions. Much higher accumulation of TNF was obtained in a constitutive system, so the wider applicability of such systems was studied. In constitutive systems, proteolytically susceptible proteins can be produced easily at low...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Monoclonal and Polyclonal Antibodies Research
Share to Protein purification and stability
Share to Viral Infectious Diseases and Gene Expression in Insects

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 12768624
DOI: 10.1002/bit.10660

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Constitutive versus thermoinducible expression of heterologous proteins in Escherichia coli based on strong PR,PL promoters from phage lambdaPMID 12768624Read ↗

Short, auto-inducible promoters for well-controlled protein expression in Escherichia coli.Semantic proximity Read ↗

Development of an Inducible Secretory Expression Vector and Host System for High Yield Production of Recombinant ProteinSemantic proximity Read ↗

Sub-cloning of Human Cytokines CXCL-10 in E Coli SystemSemantic proximity Read ↗

Soluble expression, purification, and characterization of active recombinant human tissue plasminogen activator by auto-induction in E. coliSemantic proximity Read ↗

Screening through the PLICable promoter toolbox enhances protein production in Escherichia coli.Semantic proximity Read ↗

Translational level is a critical factor for the secretion of heterologous proteins in Escherichia coli.Semantic proximity Read ↗

Exploring the 5'-UTR DNA region as a target for optimizing recombinant gene expression from the strong and inducible Pm promoter in Escherichia coli.Semantic proximity Read ↗

Escherichia coli K12 relA strains as safe hosts for expression of recombinant DNA.Semantic proximity Read ↗

Methods of preparation of recombinant cytokine proteins.Semantic proximity Read ↗

Induction of the phoE promoter upon invasion of Salmonella typhimurium into eukaryotic cells.Semantic proximity Read ↗

Construction of a plasmid vector for the regulatable high level expression of eukaryotic genes in Escherichia coli: an application to overproduction of chicken lysozyme.Semantic proximity Read ↗

Construction and characterization of new host-vector systems for the enforcement-cloning method.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.