Article

Sub-cloning of Human Cytokines CXCL-10 in E Coli System

2026-07-14

Abstract excerpt

<title>Abstract</title> <p>Competent E. coli DH5α cells were first prepared and then used for transformation with the pET M11 vector carrying a human cytokine gene. Competency was achieved through CaCl₂ treatment, which makes the bacterial cells more permeable to foreign DNA. The prepared cells were subjected to heat-shock along with the plasmid, allowed to recover in LB broth, and spread on agar plates containin...

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Literature Corpus work: d98e5649-85e7-5476-9b05-19ac0abf15c2
DOI: 10.21203/rs.3.rs-10324160/v1

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