Article
Genetic probing of the interaction between the translation factor SelB and its mRNA binding element in Escherichia coli.
Molecular & general genetics : MGG - 1 Dec 1999
Kromayer M, Neuhierl B, Friebel A, Böck A
Abstract excerpt
Decoding of the UGA codon in mRNAs for selenoproteins as selenocysteine requires interaction of the translation factor SelB with an mRNA structure, the SECIS element. A genetic analysis of this interaction was performed by selecting for intergenic suppressor mutations in selB which counteracted the detrimental effect of defined mutations in the SECIS element. Both allele-nonspecific and allele-specific mutations,...
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