Article
Antiestrogens stimulate expression of transiently transfected and endogenous genes in rat pituitary tumor cell lines.
Molecular and cellular endocrinology - 1 May 1991
Larsen P R, Warne R
Abstract excerpt
Tamoxifen, nafoxidine, and clomiphene (1 x 10(-5) M) cause 5- to 15-fold increases in transient expression of plasmids transfected into rat somatomammotrophic pituitary tumor cell lines. To be effective, the antiestrogen must be present during the calcium phosphate transfection though it does not enhance the nuclear uptake or stability of transfected plasmid. The effect occurs with mammalian (rat growth hormone,...
Topics
- Animals
- Blotting, Southern
- Gene Expression
- Growth Hormone
- Mutation
- Pituitary Gland
- Promoter Regions, Genetic
- Rats
- Tamoxifen
- Transfection
- Tumor Cells, Cultured