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Article

Glutamate-haem ester bond formation is disfavoured in flavocytochrome P450 BM3: characterization of glutamate substitution mutants at the haem site of P450 BM3.

The Biochemical journal - 14 Apr 2010

Girvan Hazel M, Levy Colin W, Williams Paul, Fisher Karl, Cheesman Myles R, Rigby Stephen E J, Leys David, Munro Andrew W

Abstract excerpt

Bacillus megaterium flavocytochrome P450 BM3 (CYP102A1) is a biotechnologically important cytochrome P450/P450 reductase fusion enzyme. Mutants I401E, F261E and L86E were engineered near the haem 5-methyl group, to explore the ability of the glutamate carboxylates to form ester linkages with the methyl group, as observed for eukaryotic CYP4 relatives. Although no covalent linkage was detected, mutants displayed...

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PMID: 20180779
DOI: 10.1042/BJ20091603

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