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Article

Flavocytochrome P450 BM3 mutant A264E undergoes substrate-dependent formation of a novel heme iron ligand set.

The Journal of biological chemistry - 28 May 2004

Girvan Hazel M, Marshall Ker R, Lawson Rachel J, Leys David, Joyce M Gordon, Clarkson John, Smith W Ewen, Cheesman Myles R, Munro Andrew W

Abstract excerpt

A conserved glutamate covalently attaches the heme to the protein backbone of eukaryotic CYP4 P450 enzymes. In the related Bacillus megaterium P450 BM3, the corresponding residue is Ala264. The A264E mutant was generated and characterized by kinetic and spectroscopic methods. A264E has an altered absorption spectrum compared with the wild-type enzyme (Soret maximum at approximately 420.5 nm). Fatty acid...

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Share to Cytochrome P-450 Enzyme System
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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 15020591
DOI: 10.1074/jbc.M401716200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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