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Article

Joining mutants of RAG1 and RAG2 that demonstrate impaired interactions with the coding-end DNA.

The Journal of biological chemistry - 10 Sept 2004

Nagawa Fumikiyo, Hirose Satoshi, Nishizumi Hirofumi, Nishihara Tadashi, Sakano Hitoshi

Abstract excerpt

In V(D)J joining of antigen receptor genes, two recombination signal sequences (RSSs), 12- and 23-RSSs, form a complex with the protein products of recombination activating genes, RAG1 and RAG2. DNaseI footprinting demonstrates that the interaction of RAG proteins with substrate RSS DNA is not ju...

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Source

Identifiers

PMID: 15249552
DOI: 10.1074/jbc.M405485200

Genes

RAG1
RAG2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026. Prepared record publication-15249552-2026-09-01.1. Manifest publication-graph-cohort-2026-09-01.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 1 Sept 2026.

Related research

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Research trail→

Click a neighbor to travelStep 1 · 12 closest

Joining mutants of RAG1 and RAG2 that demonstrate impaired interactions with the coding-end DNA.PMID 15249552Read ↗

Evidence of a critical architectural function for the RAG proteins in end processing, protection, and joining in V(D)J recombination.Semantic proximity Read ↗

Full-length RAG1 promotes contact with coding and intersignal sequences in RAG protein complexes bound to recombination signals paired in cis.Semantic proximity Read ↗

RAG proteins shepherd double-strand breaks to a specific pathway, suppressing error-prone repair, but RAG nicking initiates homologous recombination.Semantic proximity Read ↗

The DDE motif in RAG-1 is contributed in trans to a single active site that catalyzes the nicking and transesterification steps of V(D)J recombination.Semantic proximity Read ↗

Understanding how the V(D)J recombinase catalyzes transesterification: distinctions between DNA cleavage and transposition.Semantic proximity Read ↗

Autoinhibition of DNA cleavage mediated by RAG1 and RAG2 is overcome by an epigenetic signal in V(D)J recombination.Semantic proximity Read ↗

The RAG1/RAG2 complex constitutes a 3' flap endonuclease: implications for junctional diversity in V(D)J and transpositional recombination.Semantic proximity Read ↗

Role of recombination activating genes in the generation of antigen receptor diversity and beyond.Semantic proximity Read ↗

Rag mutations reveal robust alternative end joining.Semantic proximity Read ↗

RAG-1 and RAG-2-dependent assembly of functional complexes with V(D)J recombination substrates in solution.Semantic proximity Read ↗

Mutational analysis of all conserved basic amino acids in RAG-1 reveals catalytic, step arrest, and joining-deficient mutants in the V(D)J recombinase.Semantic proximity Read ↗

Hairpin coding end opening is mediated by RAG1 and RAG2 proteins.Semantic proximity Read ↗

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