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Article

RecBCD enzyme switches lead motor subunits in response to chi recognition.

Cell - 16 Nov 2007

Spies Maria, Amitani Ichiro, Baskin Ronald J, Kowalczykowski Stephen C

Abstract excerpt

RecBCD is a DNA helicase comprising two motor subunits, RecB and RecD. Recognition of the recombination hotspot, chi, causes RecBCD to pause and reduce translocation speed. To understand this control of translocation, we used single-molecule visualization to compare RecBCD to the RecBCD(K177Q) mutant with a defective RecD motor. RecBCD(K177Q) paused at chi but did not change its translocation velocity....

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Gene Expression Regulation, Bacterial

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Source

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Identifiers

PMID: 18022364
DOI: 10.1016/j.cell.2007.09.023
PMCID: PMC2151923

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Research trail→

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RecBCD enzyme switches lead motor subunits in response to chi recognition.PMID 18022364Read ↗

Intersubunit signaling in RecBCD enzyme, a complex protein machine regulated by Chi hot spots.Semantic proximity Read ↗

Translocation by the RecB motor is an absolute requirement for {chi}-recognition and RecA protein loading by RecBCD enzyme.Semantic proximity Read ↗

Chi hotspot control of RecBCD helicase-nuclease by long-range intramolecular signaling.Semantic proximity Read ↗

A RecA mutant, RecA(730), suppresses the recombination deficiency of the RecBC(1004)D-chi* interaction in vitro and in vivo.Semantic proximity Read ↗

Small-molecule sensitization of RecBCD helicase-nuclease to a Chi hotspot-activated state.Semantic proximity Read ↗

Reversible inactivation of the Escherichia coli RecBCD enzyme by the recombination hotspot chi in vitro: evidence for functional inactivation or loss of the RecD subunit.Semantic proximity Read ↗

A single mutation, RecB(D1080A,) eliminates RecA protein loading but not Chi recognition by RecBCD enzyme.Semantic proximity Read ↗

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Structures and single-molecule analysis of bacterial motor nuclease AdnAB illuminate the mechanism of DNA double-strand break resection.Semantic proximity Read ↗

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