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Article

Biochemical characterization of RecBCD enzyme from an Antarctic Pseudomonas species and identification of its cognate Chi (χ) sequence.

PloS one - 1 Jan 2018

Pavankumar Theetha L, Sinha Anurag K, Ray Malay K

Abstract excerpt

Pseudomonas syringae Lz4W RecBCD enzyme, RecBCDPs, is a trimeric protein complex comprised of RecC, RecB, and RecD subunits. RecBCD enzyme is essential for P. syringae growth at low temperature, and it protects cells from low temperature induced replication arrest. In this study, we show that the RecBCDPs enzyme displays distinct biochemical behaviors. Unlike E. coli RecBCD enzyme, the RecD subunit is...

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Identifiers

PMID: 29775464
DOI: 10.1371/journal.pone.0197476
PMCID: PMC5959072

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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Biochemical characterization of RecBCD enzyme from an Antarctic Pseudomonas species and identification of its cognate Chi (χ) sequence.PMID 29775464Read ↗

All three subunits of RecBCD enzyme are essential for DNA repair and low-temperature growth in the Antarctic Pseudomonas syringae Lz4W.Semantic proximity Read ↗

ATPase activity of RecD is essential for growth of the Antarctic Pseudomonas syringae Lz4W at low temperature.Semantic proximity Read ↗

RecD plays an essential function during growth at low temperature in the antarctic bacterium Pseudomonas syringae Lz4W.Semantic proximity Read ↗

Chi hotspot control of RecBCD helicase-nuclease by long-range intramolecular signaling.Semantic proximity Read ↗

The reduced levels of chi recognition exhibited by the RecBC1004D enzyme reflect its recombination defect in vivo.Semantic proximity Read ↗

Reversible inactivation of the Escherichia coli RecBCD enzyme by the recombination hotspot chi in vitro: evidence for functional inactivation or loss of the RecD subunit.Semantic proximity Read ↗

Intersubunit signaling in RecBCD enzyme, a complex protein machine regulated by Chi hot spots.Semantic proximity Read ↗

Translocation by the RecB motor is an absolute requirement for {chi}-recognition and RecA protein loading by RecBCD enzyme.Semantic proximity Read ↗

A RecA mutant, RecA(730), suppresses the recombination deficiency of the RecBC(1004)D-chi* interaction in vitro and in vivo.Semantic proximity Read ↗

A domain of RecC required for assembly of the regulatory RecD subunit into the Escherichia coli RecBCD holoenzyme.Semantic proximity Read ↗

A single mutation, RecB(D1080A,) eliminates RecA protein loading but not Chi recognition by RecBCD enzyme.Semantic proximity Read ↗

Construction of recB-recD genetic fusion and functional analysis of RecBDC fusion enzyme in Escherichia coli.Semantic proximity Read ↗

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