Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Chi hotspot control of RecBCD helicase-nuclease by long-range intramolecular signaling.

Scientific reports - 5 Nov 2020

Amundsen Susan K, Taylor Andrew F, Smith Gerald R

Abstract excerpt

Repair of broken DNA by homologous recombination requires coordinated enzymatic reactions to prepare it for interaction with intact DNA. The multiple activities of enterobacterial RecBCD helicase-nuclease are coordinated by Chi recombination hotspots (5' GCTGGTGG 3') recognized during DNA unwinding. Chi is recognized in a tunnel in RecC but activates the RecB nuclease, > 25 Ǻ away. How the Chi-dependent signal...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to DNA, Bacterial
Share to DNA Helicases
Share to DNA Repair

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Endonucleases

Share to Escherichia coli Proteins

Share to Exodeoxyribonuclease V

Share to Protein Structure, Quaternary

Share to Recombination, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 33154402
DOI: 10.1038/s41598-020-73078-0
PMCID: PMC7644769

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 3 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Chi hotspot control of RecBCD helicase-nuclease by long-range intramolecular signaling.PMID 33154402Read ↗

Intersubunit signaling in RecBCD enzyme, a complex protein machine regulated by Chi hot spots.Semantic proximity Read ↗

Chi hotspot activity in Escherichia coli without RecBCD exonuclease activity: implications for the mechanism of recombination.Semantic proximity Read ↗

A novel, 11 nucleotide variant of chi, chi*: one of a class of sequences defining the Escherichia coli recombination hotspot chi.Semantic proximity Read ↗

Chi hotspots trigger a conformational change in the helicase-like domain of AddAB to activate homologous recombination.Semantic proximity Read ↗

Insights into Chi recognition from the structure of an AddAB-type helicase-nuclease complex.Semantic proximity Read ↗

Reversible inactivation of the Escherichia coli RecBCD enzyme by the recombination hotspot chi in vitro: evidence for functional inactivation or loss of the RecD subunit.Semantic proximity Read ↗

RecBCD enzyme switches lead motor subunits in response to chi recognition.Semantic proximity Read ↗

Biochemical characterization of RecBCD enzyme from an Antarctic Pseudomonas species and identification of its cognate Chi (χ) sequence.Semantic proximity Read ↗

Small-molecule sensitization of RecBCD helicase-nuclease to a Chi hotspot-activated state.Semantic proximity Read ↗

A RecA mutant, RecA(730), suppresses the recombination deficiency of the RecBC(1004)D-chi* interaction in vitro and in vivo.Semantic proximity Read ↗

Nuclease activity is essential for RecBCD recombination in Escherichia coli.Semantic proximity Read ↗

A single mutation, RecB(D1080A,) eliminates RecA protein loading but not Chi recognition by RecBCD enzyme.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.