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Article

Small-molecule sensitization of RecBCD helicase-nuclease to a Chi hotspot-activated state.

Nucleic acids research - 20 Aug 2020

Karabulut Ahmet C, Cirz Ryan T, Taylor Andrew F, Smith Gerald R

Abstract excerpt

Coordinating multiple activities of complex enzymes is critical for life, including transcribing, replicating and repairing DNA. Bacterial RecBCD helicase-nuclease must coordinate DNA unwinding and cutting to repair broken DNA. Starting at a DNA end, RecBCD unwinds DNA with its fast RecD helicase on the 5'-ended strand and its slower RecB helicase on the 3'-ended strand. At Chi hotspots (5' GCTGGTGG 3'), RecB's...

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PMID: 32597964
DOI: 10.1093/nar/gkaa534
PMCID: PMC7641324

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