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Article

Optimization of the analogue-sensitive Cdc2/Cdk1 mutant by in vivo selection eliminates physiological limitations to its use in cell cycle analysis.

Open biology - 1 Jul 2014

Aoi Yuki, Kawashima Shigehiro A, Simanis Viesturs, Yamamoto Masayuki, Sato Masamitsu

Abstract excerpt

Analogue-sensitive (as) mutants of kinases are widely used to selectively inhibit a single kinase with few off-target effects. The analogue-sensitive mutant cdc2-as of fission yeast (Schizosaccharomyces pombe) is a powerful tool to study the cell cycle, but the strain displays meiotic defects, and is sensitive to high and low temperature even in the absence of ATP-analogue inhibitors. This has limited the use of...

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Identifiers

PMID: 24990387
DOI: 10.1098/rsob.140063
PMCID: PMC4118601

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Source receipt: pubmed, retrieved 7 Sept 2026.

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