Article
Random mutagenesis of Legionella pneumophila reveals genes associated with lipopolysaccharide synthesis and recognition by typing monoclonal antibodies.
Journal of applied microbiology - 1 Nov 2007
Wagner C, Krönert C, Lück P C, Jacobs E, Cianciotto N P, Helbig J H
Abstract excerpt
AIMS: To use random mutagenesis for the characterization of Legionella pneumophila lipopolysaccharide (LPS) components and serotypes. METHODS AND RESULTS: Five strains belonging to different serogroups and/or monoclonal subgroups were mutagenized using a mini-Tn10 transposon. Exactly 11 819 mutants were checked for alterations in LPS using at least 11 monoclonal antibodies (mAbs) that define L. pneumophila...
Topics
- Antibodies, Monoclonal
- Base Sequence
- Humans
- Legionella pneumophila
- Legionnaires' Disease
- Lipopolysaccharides
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Sequence Alignment
- Serotyping