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CRISPR-based mutagenesis of lipopolysaccharide biosynthesis genes in Leptospira interrogans reveals gene essentiality and confirms the role of an O-antigen polymerase.

Scientific reports - 13 Mar 2026

Fernandes Luis G V, Nally Jarlath E

Abstract excerpt

Leptospirosis is a worldwide zoonosis caused by pathogenic bacteria of the genus Leptospira. Lipopolysaccharide (LPS) is an immunodominant and protective antigen for Leptospira, but its biosynthesis remains poorly understood. In this study, we employed CRISPR/Cas9-non-homologous end-joining and CRISPR-Prime Editing to mutate key genes within the rfb locus of L. interrogans, including those involved in core...

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Identifiers

PMID: 41826615
DOI: 10.1038/s41598-026-43869-y
PMCID: PMC13111663

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