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An Enhanced System for Construction of Adenoviral Vectors by the Two-Plasmid Rescue Method

20 Mar 2000

Abstract excerpt

The two-plasmid rescue method of constructing Ad vectors, which relies on either homologous or Cre-mediated recombination between two plasmids cotransfected into 293 or 293Cre4 cells, respectively, offers advantages over other approaches because of its simplicity. We have improved the efficiency of vector construction by both homologous and Cre-mediated recombination by replacing the single ITR in the shuttle...

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PMID: 10757349
DOI: 10.1089/10430340050015590

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