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Article

Compact Bidirectional Promoters for Dual-Gene Expression in a Sleeping Beauty Transposon

4 Dec 2020

Abstract excerpt

Promoter choice is an essential consideration for transgene expression in gene therapy. The expression of multiple genes requires ribosomal entry or skip sites, or the use of multiple promoters. Promoter systems comprised of two separate, divergent promoters may significantly increase the size of genetic cassettes intended for use in gene therapy. However, an alternative approach is to use a single, compact,...

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PMID: 33291599
DOI: 10.3390/ijms21239256

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