Article
Expanding the Helicobacter pylori genetic toolbox: modification of an endogenous plasmid for use as a transcriptional reporter and complementation vector.
Applied and environmental microbiology - 1 Dec 2007
Carpenter Beth M, McDaniel Timothy K, Whitmire Jeannette M, Gancz Hanan, Guidotti Silvia, Censini Stefano, Merrell D Scott
Abstract excerpt
Helicobacter pylori is an important human pathogen. However, the study of this organism is often limited by a relative shortage of genetic tools. In an effort to expand the methods available for genetic study, an endogenous H. pylori plasmid was modified for use as a transcriptional reporter and as a complementation vector. This was accomplished by addition of an Escherichia coli origin of replication, a...
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