Article
Trinucleotide cassettes increase diversity of T7 phage-displayed peptide library.
BMC biotechnology - 5 Oct 2007
Krumpe Lauren R H, Schumacher Kathryn M, McMahon James B, Makowski Lee, Mori Toshiyuki
Abstract excerpt
BACKGROUND: Amino acid sequence diversity is introduced into a phage-displayed peptide library by randomizing library oligonucleotide DNA. We recently evaluated the diversity of peptide libraries displayed on T7 lytic phage and M13 filamentous phage and showed that T7 phage can display a more diverse amino acid sequence repertoire due to differing processes of viral morphogenesis. METHODS: In this study, we...
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