Article
Identification and characterization of mutant clones with enhanced propagation rates from phage-displayed peptide libraries.
Analytical biochemistry - 1 Oct 2014
Nguyen Kieu T H, Adamkiewicz Marta A, Hebert Lauren E, Zygiel Emily M, Boyle Holly R, Martone Christina M, Meléndez-Ríos Carola B, Noren Karen A, Noren Christopher J, Hall Marilena Fitzsimons
Abstract excerpt
A target-unrelated peptide (TUP) can arise in phage display selection experiments as a result of a propagation advantage exhibited by the phage clone displaying the peptide. We previously characterized HAIYPRH, from the M13-based Ph.D.-7 phage display library, as a propagation-related TUP resulting from a G→A mutation in the Shine-Dalgarno sequence of gene II. This mutant was shown to propagate in Escherichia...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.