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Article

T7 lytic phage-displayed peptide libraries: construction and diversity characterization.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014

Krumpe Lauren R H, Mori Toshiyuki

Abstract excerpt

In this chapter, we describe the construction of T7 bacteriophage (phage)-displayed peptide libraries and the diversity analyses of random amino acid sequences obtained from the libraries. We used commercially available reagents, Novagen's T7Select system, to construct the libraries. Using a combination of biotinylated extension primer and streptavidin-coupled magnetic beads, we were able to prepare library DNA...

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Identifiers

PMID: 24146396
DOI: 10.1007/978-1-62703-673-3_4

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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