Article
Isolation and characterization of osmosensitive vacuolar mutants of Saccharomyces cerevisiae.
Molecular microbiology - 1 Oct 1991
Latterich M, Watson M D
Abstract excerpt
The yeast vacuole plays an important role in nitrogen metabolism, storage and intracellular macromolecular degradation. Evidence suggests that it is also involved in osmohomeostasis of the cell. We have taken a mutational approach for the analysis of vacuolar function and biogenesis by the isolation of 97 mutants unable to grow if high concentrations of salt are present in the medium. Phenotypic analysis was able...
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