Article
Determination of human papillomavirus type 16 genotype and construction of cloning vector pTZ57R encoding HPV16 E7 gene.
Saudi medical journal - 1 Oct 2007
Meshkat Zahra, Soleimanjahi Hoorieh, Mahmoudi Mahmoud, Mirshahabi Hessam, Hassan Zuhair M, Ghaffari Saeed R, Sabokbar Tayebeh
Abstract excerpt
OBJECTIVE: To isolate and construct a cloning vector containing the human papillomavirus (HPV)16-E7 gene as a target for application as a DNA vaccine. METHODS: The study was performed in 2005 in Iran. The E7 gene, one of the most important HPV oncoproteins and a target molecule for therapeutic vaccines, was amplified by polymerase chain reaction (PCR). The PCR product was cloned into a suitable cloning vector and...
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