Article
[Gene characterization of E6 and E7 gene of human papillomavirus of 15 cervical cancer in Beijing].
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology - 1 Apr 2009
Ren Zhuo, Wu Hong-wei, Song Jie
Abstract excerpt
OBJECTIVE: Conducting the gene characterization of the E6 and E7 gene of human papillomavirus 16 (HPV16) isolated from 15 cases of cervical cancer at Beijing. METHODS: Overlapping primers were designed according to the full-length genomes of E6 and E7 from the GenBank and PCR was used to amplify the E6 and E7 fragments. TA clone was used to select a purified clone in order to have better and valuable sequencing...
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