Article
Time-resolved methods in biophysics. 6. Time-resolved Laue crystallography as a tool to investigate photo-activated protein dynamics.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology - 1 Oct 2007
Bourgeois Dominique, Schotte Friedrich, Brunori Maurizio, Vallone Beatrice
Abstract excerpt
When polychromatic X-rays are shined onto crystalline material, they generate a Laue diffraction pattern. At third generation synchrotron radiation sources, a single X-ray pulse of approximately 100 ps duration is enough to produce interpretable Laue data from biomolecular crystals. Thus, by initiating biological turnover in a crystalline protein, structural changes along the reaction pathway may be filmed by...
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