Article
Chromophore conformation and the evolution of tertiary structural changes in photoactive yellow protein.
Structure (London, England : 1993) - 1 Jun 2004
Anderson Spencer, Srajer Vukica, Pahl Reinhard, Rajagopal Sudarshan, Schotte Friedrich, Anfinrud Philip, Wulff Michael, Moffat Keith
Abstract excerpt
We use time-resolved crystallography to observe the structural progression of a bacterial blue light photoreceptor throughout its photocycle. Data were collected from 10 ns to 100 ms after photoactivation of the E46Q mutant of photoactive yellow protein. Refinement of transient chromophore conformations shows that the spectroscopically distinct intermediates are formed via progressive disruption of the hydrogen...
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