Article
Design of Light-Controlled Protein Conformations and Functions.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2016
Ritterson Ryan S, Hoersch Daniel, Barlow Kyle A, Kortemme Tanja
Abstract excerpt
In recent years, interest in controlling protein function with light has increased. Light offers a number of unique advantages over other methods, including spatial and temporal control and high selectivity. Here, we describe a general protocol for engineering a protein to be controllable with light via reaction with an exogenously introduced photoisomerizable small molecule and illustrate our protocol with two...
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