Article
Controlled reduction of the humidity induces a shortcut recovery reaction in the photocycle of photoactive yellow protein.
Biochemistry - 28 Jun 2005
van der Horst Michael A, van Stokkum Ivo H M, Dencher Norbert A, Hellingwerf Klaas J
Abstract excerpt
The photocycle of the blue-light photoreceptor protein Photoactive Yellow Protein (PYP) was studied at reduced relative humidity (RH). Photocycle kinetics and spectra were measured in thin films of PYP in which the relative humidity was set at values between 29 and 98% RH with saturated solutions of various salts. We show that in this range, approximately 200 water molecules per PYP molecule are released from the...
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