Article
Detection of restriction enzyme-digested target DNA by PCR amplification using a stem-loop primer: application to the detection of hypomethylated fetal DNA in maternal plasma.
Clinical chemistry - 1 Nov 2007
Tong Yu K, Chiu Rossa W K, Leung Tak Y, Ding Chunming, Lau Tze K, Leung Tse N, Lo Y M Dennis
Abstract excerpt
BACKGROUND: The discovery of cell-free fetal DNA in maternal plasma has opened up new possibilities for noninvasive prenatal diagnosis and monitoring. Among the fetal markers that have been described, methylation markers are sex and polymorphism independent. Methylation-sensitive restriction endonucleases are commonly used to digest hypomethylated DNA molecules, and the hypermethylated molecules remain intact for...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.