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Article

Establishment and Application of a Multiplex PCR NGS Method for the Genotyping of HLA-Class I and HPA.

HLA - 1 Oct 2024

He Yanmin, Wang Fang, Wu Zhipan, Zhang Wei, Zhu Faming

Abstract excerpt

Selecting compatible HLA-Class I and/or HPA platelets based on genotyping could alleviate immune platelet transfusion refractoriness (PTR). A fast and reliable method of HLA-Class I and HPA genotyping is necessary to construct a platelet donor bank with known HLA-Class I and HPA genotypes. Ten pairs of specific primers for HLA-A, HLA-B, HLA-C, HPA-1 through HPA-6w, HPA-15 and HPA-21w were designed. The...

Topics

Humans
Alleles
Antigens, Human Platelet
Blood Platelets
Genotype
Genotyping Techniques
High-Throughput Nucleotide Sequencing
Histocompatibility Antigens Class I
Histocompatibility Testing
Multiplex Polymerase Chain Reaction

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Platelet Transfusion

Source

Identifiers

PMID: 39469985
DOI: 10.1111/tan.15716

Genes

ITGA2B

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-39469985-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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