Article
Establishment and Application of a Multiplex PCR NGS Method for the Genotyping of HLA-Class I and HPA.
HLA - 1 Oct 2024
He Yanmin, Wang Fang, Wu Zhipan, Zhang Wei, Zhu Faming
Abstract excerpt
Selecting compatible HLA-Class I and/or HPA platelets based on genotyping could alleviate immune platelet transfusion refractoriness (PTR). A fast and reliable method of HLA-Class I and HPA genotyping is necessary to construct a platelet donor bank with known HLA-Class I and HPA genotypes. Ten pairs of specific primers for HLA-A, HLA-B, HLA-C, HPA-1 through HPA-6w, HPA-15 and HPA-21w were designed. The...
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