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Article

Surpassing specificity limits of nucleic acid probes via cooperativity.

The Journal of molecular diagnostics : JMD - 1 May 2010

Satterfield Brent C, Bartosiewicz Matt, West Jay A A, Caplan Michael R

Abstract excerpt

The failure to correctly identify single nucleotide polymorphisms (SNPs) significantly contributes to the misdiagnosis of infectious disease. Contrary to the strategy of creating shorter probes to improve SNP differentiation, we created larger probes that appeared to increase selectivity. Specifically, probes with enhanced melting temperature differentials (>13x improvement) to SNPs were generated by linking two...

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Identifiers

PMID: 20304941
DOI: 10.2353/jmoldx.2010.090056
PMCID: PMC2860473

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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