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Article

Real-time PCR genotyping using displacing probes.

Nucleic acids research - 15 Apr 2004

Cheng Jinping, Zhang Yongyou, Li Qingge

Abstract excerpt

Simple and reliable genotyping technology is a key to success for high-throughput genetic screening in the post-genome era. Here we have developed a new real-time PCR genotyping approach that uses displacement hybridization-based probes: displacing probes. The specificity of displacing probes could be simply assessed through denaturation analysis before genotyping was implemented, and the probes designed with...

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Identifiers

PMID: 15087493
DOI: 10.1093/nar/gnh055
PMCID: PMC407835

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