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Article

Site-specific incorporation of tryptophan analogues into recombinant proteins in bacterial cells.

Journal of the American Chemical Society - 29 Aug 2007

Kwon Inchan, Tirrell David A

Abstract excerpt

A designed yeast phenylalanyl-tRNA synthetase (yPheRS (T415G)) activates four tryptophan (Trp) analogues (6-chlorotryptophan (6ClW), 6-bromotryptophan (6BrW), 5-bromotryptophan (5BrW), and benzothienylalanine (BT)) that are not utilized by the endogenous E. coli translational apparatus. Introduction of yPheRS (T415G) and a mutant yeast phenylalanine amber suppressor tRNA (ytRNAPheCUA_UG) into an E. coli...

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PMID: 17685515
DOI: 10.1021/ja071773r

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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