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Article

Affinity-matured recombinant antibody fragments analyzed by single-molecule force spectroscopy.

Biophysical journal - 15 Nov 2007

Morfill Julia, Blank Kerstin, Zahnd Christian, Luginbühl Beatrice, Kühner Ferdinand, Gottschalk Kay-E, Plückthun Andreas, Gaub Hermann E

Abstract excerpt

For many applications, antibodies need to be engineered toward maximum affinity. Strategies are in demand to especially optimize this process toward slower dissociation rates, which correlate with the (un)binding forces. Using single-molecule force spectroscopy, we have characterized three varian...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 17675348
DOI: 10.1529/biophysj.107.112532
PMCID: PMC2072072

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-17675348-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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