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Article

Characterization of monobody scaffold interactions with ligand via force spectroscopy and steered molecular dynamics.

Scientific reports - 4 Feb 2015

Cheung Luthur Siu-Lun, Shea Daniel J, Nicholes Nathan, Date Amol, Ostermeier Marc, Konstantopoulos Konstantinos

Abstract excerpt

Monobodies are antibody alternatives derived from fibronectin that are thermodynamically stable, small in size, and can be produced in bacterial systems. Monobodies have been engineered to bind a wide variety of target proteins with high affinity and specificity. Using alanine-scanning mutagenesis simulations, we identified two scaffold residues that are critical to the binding interaction between the monobody...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 25650239
DOI: 10.1038/srep08247
PMCID: PMC4316159

Genes

MBP

Variants

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